Martina McBride and Kelly Clarkson's Rock the Room Tour, to benefit Musicians on Call, kicked off in Nashville at City Winery on Wednesday night (Oct. 21). One of the night's biggest highlights was a duet between McBride and Reba McEntire; the two performed one of the redhead's most popular songs, "Does He Love You?"

"You'll recognize it, I hope," McEntire told the crowd to introduce the tune. "We've done it together before."

"Does He Love You?" originally featured McEntire and Linda Davis. It was released as a single in August of 1993 and hit No. 1. The crowd immediately began cheering when the song began; its recognizable melody is impossible to miss, and the lighthearted banter between McEntire and McBride was highly entertaining.

Right before they launched into the duet, McBride announced, "I don't think it would be quite right unless we got Shane [Tarleton] here onstage."

Tarleton, Warner Music Nashville's creative director, was honored at the show with the "Golden Ukulele," for his leadership and volunteer work with Musicians on Call. The two singers gleefully made him uncomfortable as they battled it out for his love during the song. Press play on the video above to watch the performance.

McBride and Clarkson will continue on to New York City, Los Angeles and Washington, D.C., for the Rock the Room Tour. The tour will raise funds for Musicians on Call, a non-profit that offers live and recorded music to patients in healthcare facilities nationwide, as well as raise awareness of music's healing impact and honor leaders in entertainment.

More information about the Rock the Room Tour is available on Musicians on Call’s website.

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