The 2016 American Country Countdown Awards will open with a collaborative performance featuring three country women: Martina McBride, Jennifer Nettles and Cam. The three singers will perform McBride's "This One's for the Girls."

Written by Chris Lindsey, Hillary Lindsey and Aimee Mayo, "This One's for the Girls" became a Top 5 hit on the country charts following its release in 2003; it also hit No. 1 on the Billboard Adult Contemporary chart. The song was the first single from McBride's Martina album, and featured background vocals from Carolyn Dawn Johnson, Faith Hill and McBride's daughters Delaney and Emma.

The trio's awards show-opening performance will be their first time onstage together.

"I always look forward to singing this song because it resonates with so many different people of all different ages,” McBride says in a press release. “With Jennifer and Cam joining me for the first time, the song will be even more fun, and I’m so happy we’ll be performing this together."

Also performing at the 2016 ACC Awards are Toby Keith, Brooks & DunnKelsea Ballerini, Dan + Shay, Sam HuntCole SwindellLuke BryanFlorida Georgia LineThomas RhettChris Janson and Carrie Underwood. Not much is known about any of the performances, but event organizers have revealed that Keith will be playing in tribute to the late Merle Haggard; Haggard’s backing band, the Strangers, will join him onstage.

Presenters at the 2016 ACC Awards include Chase Bryant, Maddie & Tae, Paula Abdul, Terry Crews, Cat Deeley, Sara Evans, Trent Harmon, Jana Kramer, Chris Lane, Scotty McCreery, Nettles, Michael Ray, Canaan Smith, Luke Wilson and Dwight Yoakam. Matthew McConaughey will be introducing Keith’s performance, and Reba McEntire will help honor good friends Brooks & Dunn during their Nash Icon Award presentation.

After being postponed late in 2015, the American Country Countdown Awards are set to return on May 1. The show will air live on Fox from 8PM ET until 10PM ET, from the Forum in Inglewood, Calif. The two-hour awards ceremony will be the first country music awards show to air live from Los Angeles in more than 10 years.

See Photos from the 2014 American Country Countdown Awards